Biol. Pharm. Bull. 28(1) 181—184 (2005)
نویسندگان
چکیده
ment of acquired, refractory and fatal diseases in addition to inheritable gene deficiency diseases. Moreover, its application range is spreading to acute diseases or traumas. The gene delivery systems in vivo can be categorized as viral and nonviral approaches. Safety in usage of viral vectors for clinical gene therapy is not yet sufficient, whereas plasmid DNA (pDNA), which is a typical nonviral vector, has advantages in safety compared with a viral vector. Recently, gene transfection efficiency using nonviral vectors has improved due to the development of various gene carriers such as cationic liposome. However, transfection utilizing naked pDNA is the simplest and safest nonviral gene delivery system since naked pDNA can be used without consideration of cytotoxicity by gene carrier. When the genes were administered by the vasculature route, they were distributed to the whole body via the bloodstream, leading to inadequate organ-selective or diseased site-selective gene delivery, and were rapidly degraded by reticuloendothelial cells (liver Kupffer cell, etc.) and nuclease in blood. Although it was previously reported that the organ-selective gene transfection using naked pDNA had been achieved by direct injection, electroporation, gene gun and so on, there is great concern about safety because physical forces against the organs are required; consequently, the continuous administration of pDNA is limited. We previously developed a method of application of drugs to the surface of intraperitoneal organs such as kidney, liver, stomach and intestine, and found it to be a useful method for site-selective drug delivery to these organs. Furthermore, we reported on liverand lobe-selective gene expression following the instillation of naked pDNA to the liver surface in mice. Kidney-targeted gene therapy is expected to treat fatal diseases, including renal cell carcinoma, chronic renal fibrosis and glomerulonephritis. Therefore, the present study was undertaken to elucidate the unilateral kidney-selective gene transfer following the administration of naked pDNA to the surface of mouse kidney. MATERIALS AND METHODS
منابع مشابه
Biol. Pharm. Bull. 28(3) 563—564 (2005)
Tomomi NOGUCHI, Chihiro SHINJI, Hisayoshi KOBAYASHI, Makoto MAKISHIMA, Hiroyuki MIYACHI, and Yuichi HASHIMOTO* Institute of Molecular & Cellular Biosciences, The University of Tokyo; 1–1–1 Yayoi, Bunkyo-ku, Tokyo 113–0032, Japan: and Department of Biochemistry, Nihon University, School of Medicine; 30–1 Oyaguchi-kamicho, Itabashi-ku, Tokyo 173–8610, Japan. Received January 13, 2005; accepted Ja...
متن کاملBiol. Pharm. Bull. 28(10) 2026—2027 (2005)
Shuso TAKEDA, Yuji ISHII, Peter I. MACKENZIE, Kiyoshi NAGATA, Yasushi YAMAZOE, Kazuta OGURI, and Hideyuki YAMADA* a Graduate School of Pharmaceutical Sciences, Kyushu University; Fukuoka 812–8582, Japan: b Department of Clinical Pharmacology, Flinders Medical Centre and Flinders University; Adelaide, SA5042, Australia: c Graduate School of Pharmaceutical Sciences, Tohoku University; Sendai 980–...
متن کاملBiol. Pharm. Bull. 28(5) 937—939 (2005)
a Laboratory of Medicinal Pharmacognosy, Tokyo University of Pharmacy and Life Science, School of Pharmacy, 1432–1 Horinouchi, Hachioji, Tokyo 192–0392, Japan: b Functional Foods Development Division, Kaneka Corporation; Takasago, Hyogo 676–8688, Japan: c Life Science Research Laboratories, Life Science RD Center, Kaneka Corporation; Takasago, Hyogo 676–8688; Japan: d Division of Molecular Meta...
متن کاملBiol. Pharm. Bull. 28(2) 374—377 (2005)
cales) are known to contain structurally unique secondary metabolites, such as plastoquinones, chromanols, a cyclopentenone, and polysaccharides. These compounds show various biological activities due to their unique structure. We previously reported that the methanolic extract of the brown alga, Sargassum micracanthum (KUETZING) ENDLICHER, “Togemoku” in Japanese, showed strong antioxidant acti...
متن کاملBiol. Pharm. Bull. 28(4) 768—771 (2005)
Rat pheochromocytoma PC12 cells undergo neuronal differentiation in response to nerve growth factor. We show here that exposure of PC12 cells to Nardostachys chinensis glycoside induces the outgrowth of neurites, increases the activity of AChE, triggers cell cycle arrest in G1 and enhances the expression of growth associated protein 43 (GAP-43). Both the outgrowth of neurites and the increase i...
متن کاملAntiinflammatory Constituents of Teramnus labialis
1. Alagarsamy, V., Raja Salomon, V., Vanikavitha, G., Paluchamy, V., Ravichandran, M., Arnold Sujin, A., Thangathirupathy, A., Amuthalakshmi, S. and Revathi R., Biol. Pharm. Bull., 2002, 25, 1432. 2. Alagarsamy, V., Muthukumar, V., Pavalarani, N., Vasanthanathan, P. and Revathi R., Biol. Pharm. Bull., 2003, 26(4), 557. 3. Chaurasia, M.R. and Sharma, S.K., Arch. Pharm., 1982, 315, 377. 4. Manabu...
متن کامل